Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. I. Specimen handling techniques.
نویسندگان
چکیده
منابع مشابه
Application of scanning electron microscopy to x-ray analysis of frozen- hydrated sections. I. Specimen handling techniques
X-ray microanalysis of frozen-hydrated tissue sections permits direct quantitative analysis of diffusible elements in defined cellular compartments. Because the sections are hydrated, elemental concentrations can be defined as wet-weight mass fractions. Use of these techniques should also permit determination of water fraction in cellular compartments. Reliable preparative techniques provide fl...
متن کاملApplication of scanning electron microscopy to x-ray analysis of frozen- hydrated sections. II. Analysis of standard solutions and artificial electrolyte gradients
New specimen handling and analytic techniques for the application of x-ray microanalysis to studies of cell and organ biology have been recently described (Saubermann et al., 1981, J. Cell Biol. 88:257-267). Based on these techniques, absolute quantitative standardization has been established through x-ray analysis of frozen-hydrated and then dried sections of independently measured standard so...
متن کاملApplication of scanning electron microscopy to x-ray analysis of frozen- hydrated sections. III. Elemental content of cells in the rat renal papillary tip
The electrolyte and water content of cellular and interstitial compartments in the renal papilla of the rat was determined by x-ray microanalysis of frozen-hydrated tissue sections. Papillae from rats on ad libitum water were rapidly frozen in a slush of Freon 12, and sectioned in a cryomicrotome at -30 to -40 degrees C. Frozen 0.5-micrometer sections were mounted on carbon-coated nylon film ov...
متن کاملElectron microscopy of frozen hydrated biological specimens.
The use of frozen hydrated specimens for molecular structure determination is limited primarily by radiation damage. The radiation damage effect in frozen hydrated catalase crystals has been measured in terms of the loss of electron diffraction. The results show an improvement for this type or:specimen relative to wet hydrated or to glucose embedded catalase crystals at room temperature. Bright...
متن کاملNano-structural Characterization of Post-annealed ZnO Thin Films by X-ray Diffraction and Field Emission Scanning Electron Microscopy
ZnO thin films were deposited on Si(400) substrates by e-beam evaporation technique, and then post-annealed at different annealing temperatures (200-800°C). Dependence of the crystallographic structure, nano-strain, chemical composition and surface physical Morphology of these layers on annealing temperature were studied. The crystallographic structure of films was studied using X-Ray Diffracti...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1981
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.88.2.257